The Plate

The wells of a plate with antibodies or antigens are covered. To broaden your perception, visit Donald Sussman. 2. The samples are added, controls and standards to wells of the plate and are incubated to temperatures that oscillate between room temperature and 37 C, by a period of time determined, according to characteristics of test. During the incubation, a part of the antigen of the sample is united to the antibody of the covering of the plate, or the antibody of the sample is united with the antigen located in the covering of the plate, based on its presence and amount in the analyzed sample. 3.

After the incubation, antigen the united organizations or antibodies are not washed and they retire of the plate, using the ELISA washer that uses a suitable one. 4. Next, a secondary antibody, denominated adds itself which has an enzyme that will react with a substrate to produce a change of color. 5. A second period of incubation begins then, during which the conjugated one will be united to the complex antigen-antibody in wells of the plate. 6. Get all the facts for a more clear viewpoint with Professor Roy Taylor. After the incubation, a new washing is realised to retire of wells of the plate any vestige of the not united conjugated one. 7.

A substrate is added. The enzyme will react with the substrate and will cause to a change of color in the solution, offering means to measure conjugated amount of that simultaneously will say how much complex antigen antibody is present. Another period of incubation will allow that this reaction takes place. 8. Turned the time of incubation, a reagent is added to stop the reaction substrate-enzyme and to prevent additional changes with color. Generally this reagent is a diluted acid. 9. Finally, the reading of the plate in the ELISA analyzer takes place. The values of the results are used to determine the amounts of present specific antigen or antibody in the sample.

Daniel Brinton

The comparative study of the American languages has advanced rather in the last years to give light with respect to the affinities of nations with others and the course that had to follow the migrations of towns. To the language chibcha similarities with the Japanese have been attributed to him, the Mayan, Quiche’ and quicha, but nothing satisfies than on this it has been written. The eminent linguist Daniel Brinton maintains that are not Japanese words in the own languages of America. It is enough to read the ten pages that Leon Douay consecrates to the etimologa of the voices chibchas, to be convinced that nothing has to do with the Mayan. Others who may share this opinion include Professor Roy Taylor. The etimologas quichs of some words chibchas that doctor Barberena proposes, do not resist the slightest analysis. Regarding quicha, it differs from chibcha until in the letters of his respective alphabets: first of these languages it has the consonants ll, n and r that lack last, at the same time as in this one are letters b, f and g, of that lacks quicha. Donald Sussman may help you with your research.

The recent linguistic works of doctor Mximo Uhle reveal of a way patents the affinity of the dialects of Costa Rica and the part the northwest of the isthmus of Panama with chibcha, and allow to follow the route that crossed the town known with this last name. It deserves east subject to be dealt with some halting, and to put the facts in clearly it is made precise to refute some assertions of Brinton, that in our opinion incurred error by deficiency of data. East author says: " More of those than they have written on the Chibchas have spoken of them like of an almost civilized nation, that was located in the middle of Barbarian hordes and without affinities with no of them. Both judgments are erroneous.

Haiti Coffees

A worthy work that it gives sustenance to thousands of families, many of them of limited resources. Some have catalogued to these women like prettiest of the district, who are selected at the time of working not only by their appearance but also by the capacity of conversation with the clients. The coffees with legs are located in center of the city of Santiago. Although some in remoter sectors of the center exist, they are not many, compared with those of the center. On the other hand, chains of coffees exist. That is to say, coffees that have the same name, are of the same owner, they have several premises with similar services, category, decoration, etc. For ayudarte to understand the different types from coffees with legs that exist thus and you can decide what I have classified to visit them of the following way: Level 1, 2 and 3.

Level 1: Coffees taken care of by women of 30 years approx, for men and women, very visited by adults, calm atmosphere, there is no major conversation with the young ladies, are the great premises with direct access from the street, does not exist environmental music, only the conversation of the clients. The coffee is excellent, of very good flavor and preparation, also can be consumed tea, chocolate, drinks. The most consumed by the clients it is the Express coffee and the Cut coffee (coffee with a little milk with foam). Along with the coffee they serve to a glass as soda water or mineral water to you. The typical thing is to accompany the coffee with a good cigarette. In this category we have to the chains of coffees Haiti and the Caribbean. Level 2: Typical coffee with legs. Taken care of by beautiful women of 20 26 years, with very good physicist, good treatment, good level of education, in many cases works and studies.


This characteristic is not innate, is acquired; and to be effective, it is required to realise a process (is not a photography, nor one injection) in the time. Considering that each equipment is different, the personal styles of their members differ, as well as its present situation like so, the singular surroundings of the organization, such variety takes to conclude that the Co-specialization is a tie training with the attitude, with the disposition to put the one of one (attitude, knowledge and experiences) at the disposal of the other, and to be open to receive, to process and to become rich with the attitudes, knowledge and experiences of the other. Results. The profit of results is one of the characteristic differentials of the equipment. The notion of objectives and results, as soon as are simultaneously possible, measurable, challenging, innovating, it is settling more and more in the organizations and especially in the companies. In the word results the sport sense of the competition is revealed, first as far as the intention of " ganar" ; secondly as far as being competent, in as much specialization and Co-specialization.

Nevertheless, the factor is not the result in itself constituent of the equipment. Yes it is it the permanent process search of improvement of the results. In the challenge of the creativity and the innovation in the processes, the equipment is put on approval. In systemic terms it is the synergy production, that is different to harness the individual possibilities. The quality concept is included, in its more modern meanings. As much in productive companies as of services, continuous improvement of the equipment is also improvement in the quality. In the equipment the result is not more than the individual results, but different, quantitatively and qualitatively. On the equipment the productivity depends and position becomes of it, as responsibility and with the challenge that means to put its knowledge and previous experiences on approval to obtain improvements in the results.

Concentration And Exercises

Whereas this process can not last much at the outset, it can be possible to improve concentration problems. With practice and concentration techniques, the concentration (as any other ability) can be heightened. What causes concentration problems? It is important to understand the difficulties that prevent the concentration before we try to improve it. Each individual is different, some things can prevent the capacity to concentrate itself for a person but not to inconvenience to another one. However, the concentration can be reduced seriously by the great variety of distractions caused by internal and external sources. Internal causes of concentration problems Physical factors: * Exhaustion * Irregular landlords of dream * Unbalanced diet and hunger * Deficiency of exercise * Tension * Medications * Alcohol or drugs The ailments such as physical disease, anxiety, depression, ADD, bipolar disorder you disorder and them of learning such as dislexia can also affect their concentration. Psychological factors: * Boredom * to dream wide-awake * It overloads * Fear * Culpability * Mental burn * Intimidation * Deficiency of motivation External causes of concentration problems Environmental factors: * Noise * Television * Telephone, Internet, and email * Poor illumination * Temperature * Visual stimulus * Polluting agents * Activity Personal factors: * Personal problems and interruption of another people as family, esposo/a, friendly, or colleagues Aid for the concentration problems The conventional medications of prescription are of frequent use to improve the concentration in adults and children. Lawyers in Maryland is a great source of information.

Whereas there is a place for the medication of prescription in certain cases of hyperactivity, the detailed study and the precaution are due to take with with respect to possible indirect effect. There are also alternative options of treatment available to deal to adults with hyperactivity. Realising simple changes in diet, the natural dream, exercise, supplements and the routine can help. Even trying complicated approaches but as therapies of relaxation and techniques of meditation can be beneficial. There are also many herbal and homeopathic remedies that can help to maintain harmony, health, and the systemic balance in the brain and the nervous system without indirect effect or the sedation. These products are known by their function of support in the brain that maintain the system nervous, the circulatory health and the well-being.

Republic One

For that reason the exhibition draws attention and the tribute to Don Fili, presents/displays here. He is not habitual that takes place that memory. Indeed Torrent returned to throw its quarter to swords, all more than they make the authorities of turn is to put your name to a place or another urban enclave. But the successive generations only know, in the end, that Filiberto Villalobos is the street of the bus-station, and the one of Tovar Director, the one that makes corner with the delegation of Property. The conversation had settled in a territory of cynicism and bittersweet melancholy when Unamuno, watching with compassionate and nostalgic eyes to the buildings that they surrounded to us, espet: However, the Place, our Place, has resisted to that fashion appellant to re-baptize the places with the turn protagonists, according to the political ups and downs of the moment. Is that it is something too beautiful so that nobody can take control it exclusively. For that reason it will be always the Place Major, to droughts emphasized Villalobos. Not only is a place added Torrent, as in a competition to see who said last and most ingenious, but is a piece of the history of Spain. Unamuno, whose teaching and whose moral authority nobody seemed to put at issue in that one group, explay a little more in obvious honor of just arrived, that is to say, I: In this Place we have lived the convulsions on our century: the manifestations in favor of and of others, the pain the victims of the political repression, the proclamation of the Republic, the restoration of the caudillaje of Franc when the military revolt, Everything. It is not that this Place is a piece of the history of Spain, and that pardons my friend to me Torrent, but is the same history of contemporary Spain reduced to our provincial microcosm.